A semen culture is a laboratory test that detects bacteria or other disease-causing microorganisms in semen to diagnose infections of the male urogenital tract. The sample is collected by masturbation into a sterile container after 3–5 days of sexual abstinence and should be analysed as soon as possible. If the result is positive, an antibiotic susceptibility test is performed to identify the most effective antibiotic treatment. A negative result does not completely rule out infection.